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rabbit polyclonal primary anti trpc6 antibody  (Danaher Inc)


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    Danaher Inc rabbit polyclonal primary anti trpc6 antibody
    Rabbit Polyclonal Primary Anti Trpc6 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+primary+anti+trpc6+antibody/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pm23700295-68-5-10
    Average 99 stars, based on 20243 article reviews
    rabbit polyclonal primary anti trpc6 antibody - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Expression of transient receptor potential channel 6 in cervical cancer
    Article Snippet: .. After electrophoretic transfer of the proteins to a polyvinylidene difluoride membrane (Millipore, Bedfordshire, UK) using a MiniProtein III system (Bio-Rad), immunoblotting was performed using rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:2000, Abcam, Cambridge, UK) and primary anti-β-actin (diluted 1:4000, Santa Cruz Biotechnologies, Santa Cruz, CA), and developed in an enhanced chemiluminescence system (Pierce) using specific peroxidase-conjugated anti-IgG secondary antibodies (1:4000 dilution, Santa Cruz). .. For quantification purposes, densitometric measurements were performed using the Quantity One image analysis software for Windows ( Bio-Rad).

    Article Title: Expression of transient receptor potential channel 6 in cervical cancer
    Article Snippet: .. After electrophoretic transfer of the proteins to a polyvinylidene difluoride membrane (Millipore, Bedfordshire, UK) using a MiniProtein III system (Bio-Rad), immunoblotting was performed using rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:2000, Abcam, Cambridge, UK) and primary anti-β-actin (diluted 1:4000, Santa Cruz Biotechnologies, Santa Cruz, CA), and developed in an enhanced chemiluminescence system (Pierce) using specific peroxidase-conjugated anti-IgG secondary antibodies (1:4000 dilution, Santa Cruz). .. For quantification purposes, densitometric measurements were performed using the Quantity One image analysis software for Windows (Bio-Rad).

    Western Blot:

    Article Title: Expression of transient receptor potential channel 6 in cervical cancer
    Article Snippet: .. After electrophoretic transfer of the proteins to a polyvinylidene difluoride membrane (Millipore, Bedfordshire, UK) using a MiniProtein III system (Bio-Rad), immunoblotting was performed using rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:2000, Abcam, Cambridge, UK) and primary anti-β-actin (diluted 1:4000, Santa Cruz Biotechnologies, Santa Cruz, CA), and developed in an enhanced chemiluminescence system (Pierce) using specific peroxidase-conjugated anti-IgG secondary antibodies (1:4000 dilution, Santa Cruz). .. For quantification purposes, densitometric measurements were performed using the Quantity One image analysis software for Windows ( Bio-Rad).

    Article Title: Expression of transient receptor potential channel 6 in cervical cancer
    Article Snippet: .. After electrophoretic transfer of the proteins to a polyvinylidene difluoride membrane (Millipore, Bedfordshire, UK) using a MiniProtein III system (Bio-Rad), immunoblotting was performed using rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:2000, Abcam, Cambridge, UK) and primary anti-β-actin (diluted 1:4000, Santa Cruz Biotechnologies, Santa Cruz, CA), and developed in an enhanced chemiluminescence system (Pierce) using specific peroxidase-conjugated anti-IgG secondary antibodies (1:4000 dilution, Santa Cruz). .. For quantification purposes, densitometric measurements were performed using the Quantity One image analysis software for Windows (Bio-Rad).

    Article Title: The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells
    Article Snippet: Equal amounts of each protein sample (80 μg) were separated by SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Invitrogen) using a wet transfer. .. Immunoblotting was performed with a rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:500, Abcam, Cambridge, UK), a primary rabbit anti-human c-MET polyclonal antibody (diluted 1:50, Thermo Fisher Scientific, Cheshire, UK) or a goat polyclonal primary anti-β-actin (diluted 1:1 000, Santa Cruz, CA, USA) and then developed with the enhanced chemiluminescence system (Amersham, Uppsala, Sweden) using specific peroxidase-conjugated anti-IgG secondary antibodies. .. The bands were quantified by densitometry using ImageJ software (NIH, Bethesda, MD, USA), and the values are shown as ratios.

    Article Title: Expression of TRPC6 in benign and malignant human prostate tissues
    Article Snippet: Equal amounts of each protein sample (40 μg) were separated by electrophoresis on sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Invitrogen) using a semi-dry electroblotter. .. Immunoblotting was performed using rabbit polyclonal primary anti-TRPC6 antibody (diluted 1:1 000, Abcam) and goat polyclonal primary anti-β-actin (diluted 1:1 000, Santa Cruz, CA, USA), and developed in the enhanced chemiluminescence system (ECL, Amersham, Uppsala, Sweden) using specific peroxidase-conjugated anti-IgG secondary antibodies. ..

    Article Title: Critical role of TRPC6 channels in the development of human renal cell carcinoma.
    Article Snippet: Renal cell carcinoma (RCC) is the most common tumor arising from the cells in the lining of tubules in the kidney.. Some members of the Ca-permeable transient receptor potential canonical (TRPC) family of channel proteins have demonstrated a role in the proliferation of some types of cancer cells.. In this study, we investigated the role of TRPC6 in the development of human RCC.

    Incubation:

    Article Title: Expression of transient receptor potential channel 6 in cervical cancer
    Article Snippet: .. Sections were incubated at 4°C overnight in a 1:800 dilution of the rabbit polyclonal primary anti-TRPC6 antibody (Abcam). ..

    Staining:

    Article Title: Critical role of TRPC6 channels in the development of human renal cell carcinoma.
    Article Snippet: Renal cell carcinoma (RCC) is the most common tumor arising from the cells in the lining of tubules in the kidney.. Some members of the Ca-permeable transient receptor potential canonical (TRPC) family of channel proteins have demonstrated a role in the proliferation of some types of cancer cells.. In this study, we investigated the role of TRPC6 in the development of human RCC.



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    primary polyclonal rabbit anti trpc6 antibody  (Alomone Labs)
    94
    Alomone Labs primary polyclonal rabbit anti trpc6 antibody
    Effects of the TRPC3/C6/C7 inhibitor, knockdown of TRPC3/C6, and Ca 2+ chelator on ASO uptake, and examination of L687-mediated uptake pathways. ( A ) ASO uptake was analysed by incubating cells with or without a TRPC inhibitor (SKF96365). Alexa647-AmNA#26 (10 nM) was added to either 10 μM L687, 20 μM GSK1702934A, or 30 μM CBD with or without 20 μM SKF96365 in the medium. After 24 h, the intracellular fluorescence intensities were analysed by flow cytometry. Data are shown as the relative MFI of ASO in DMSO. All data are presented as mean ± standard error of the mean (SEM) of three independent experiments ( n = 3). Statistical significance was determined by comparing with values of DMSO using Tukey's test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ( B ) siRNA-mediated knockdown of the TRPC3/C6 channels. TRPC3 siRNA (30 nM) or <t>TRPC6</t> siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed and compared with that in the untreated cells. ( C ) siRNA-mediated knockdown of TRPC3/C6 channel. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed by western blot and compared with that in untreated cells. ( D ) The effects of siRNA-mediated TRPC3/C6 channel knockdown on ASO uptake. TRPC3 siRNA (30 nM), TRPC6 (10 nM), or a combination of both were transfected into A549 cells for 48 h. The medium was replaced with Alexa647-AmNA#26 containing L687, and intracellular fluorescence intensities were analysed after 24 h. Data are shown as the relative MFI of ASO in DMSO. ( E ) Analysis of ASO uptake after incubating cells with a Ca 2+ chelator (BAPTA-AM). ASO and L687 were added to the medium, with or without 10 μM BAPTA-AM. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( F ) Analysis of dextran uptake mediated by L687. Alexa647-labelled dextran (1 and 3 μM) with 10 and 30 μM L687 was added to the medium, and A549 cells were cultured for 24 h. Intracellular fluorescence was analysed by flow cytometry, and the relative MFI was compared with 1 μM of Alexa647-dextran with DMSO. ( G ) Analysis of ASO uptake by incubating the cells with a macropinocytosis inhibitor (Cytochalasin D). L687 (30 μM) was then added to the medium. The following day, cells were then washed twice with PBS and incubated with cytochalasin D in the medium for 1 h. Then, cells were washed twice with PBS and incubated with Alexa647-AmNA#26 (10 nM) and L687 (30 μM). After 4 h, intracellular fluorescence intensities were analysed by flow cytometry. ( H ) Analysis of ASO uptake by incubating cells with a macropinocytosis inhibitor (EIPA). ASO and L687 were added to the medium with or without 100 μM EIPA. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( I ) Fluorescence imaging analysis of ASO incorporated into cells. Alexa647-AmNA#26 (100 nM) and L687 (30 μM) were added to the medium, and staining with Lysotracker-green and Hoechst, fluorescence microscopy imaging, and image analysis were performed after 48 h. ASO, antisense oligonucleotide; CBD, cannabidiol; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; TRPC, transient receptor potential canonical.
    Primary Polyclonal Rabbit Anti Trpc6 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+primary+anti+trpc6+antibody/Anti-TRPC6+(extracellular)+Antibody/pmc11109983-106-12-18
    Average 94 stars, based on 1 article reviews
    primary polyclonal rabbit anti trpc6 antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    rabbit polyclonal primary anti trpc6 antibody  (Danaher Inc)
    99
    Danaher Inc rabbit polyclonal primary anti trpc6 antibody
    Effects of the TRPC3/C6/C7 inhibitor, knockdown of TRPC3/C6, and Ca 2+ chelator on ASO uptake, and examination of L687-mediated uptake pathways. ( A ) ASO uptake was analysed by incubating cells with or without a TRPC inhibitor (SKF96365). Alexa647-AmNA#26 (10 nM) was added to either 10 μM L687, 20 μM GSK1702934A, or 30 μM CBD with or without 20 μM SKF96365 in the medium. After 24 h, the intracellular fluorescence intensities were analysed by flow cytometry. Data are shown as the relative MFI of ASO in DMSO. All data are presented as mean ± standard error of the mean (SEM) of three independent experiments ( n = 3). Statistical significance was determined by comparing with values of DMSO using Tukey's test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ( B ) siRNA-mediated knockdown of the TRPC3/C6 channels. TRPC3 siRNA (30 nM) or <t>TRPC6</t> siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed and compared with that in the untreated cells. ( C ) siRNA-mediated knockdown of TRPC3/C6 channel. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed by western blot and compared with that in untreated cells. ( D ) The effects of siRNA-mediated TRPC3/C6 channel knockdown on ASO uptake. TRPC3 siRNA (30 nM), TRPC6 (10 nM), or a combination of both were transfected into A549 cells for 48 h. The medium was replaced with Alexa647-AmNA#26 containing L687, and intracellular fluorescence intensities were analysed after 24 h. Data are shown as the relative MFI of ASO in DMSO. ( E ) Analysis of ASO uptake after incubating cells with a Ca 2+ chelator (BAPTA-AM). ASO and L687 were added to the medium, with or without 10 μM BAPTA-AM. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( F ) Analysis of dextran uptake mediated by L687. Alexa647-labelled dextran (1 and 3 μM) with 10 and 30 μM L687 was added to the medium, and A549 cells were cultured for 24 h. Intracellular fluorescence was analysed by flow cytometry, and the relative MFI was compared with 1 μM of Alexa647-dextran with DMSO. ( G ) Analysis of ASO uptake by incubating the cells with a macropinocytosis inhibitor (Cytochalasin D). L687 (30 μM) was then added to the medium. The following day, cells were then washed twice with PBS and incubated with cytochalasin D in the medium for 1 h. Then, cells were washed twice with PBS and incubated with Alexa647-AmNA#26 (10 nM) and L687 (30 μM). After 4 h, intracellular fluorescence intensities were analysed by flow cytometry. ( H ) Analysis of ASO uptake by incubating cells with a macropinocytosis inhibitor (EIPA). ASO and L687 were added to the medium with or without 100 μM EIPA. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( I ) Fluorescence imaging analysis of ASO incorporated into cells. Alexa647-AmNA#26 (100 nM) and L687 (30 μM) were added to the medium, and staining with Lysotracker-green and Hoechst, fluorescence microscopy imaging, and image analysis were performed after 48 h. ASO, antisense oligonucleotide; CBD, cannabidiol; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; TRPC, transient receptor potential canonical.
    Rabbit Polyclonal Primary Anti Trpc6 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+primary+anti+trpc6+antibody/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pm23700295-68-5-10
    Average 99 stars, based on 1 article reviews
    rabbit polyclonal primary anti trpc6 antibody - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

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    Effects of the TRPC3/C6/C7 inhibitor, knockdown of TRPC3/C6, and Ca 2+ chelator on ASO uptake, and examination of L687-mediated uptake pathways. ( A ) ASO uptake was analysed by incubating cells with or without a TRPC inhibitor (SKF96365). Alexa647-AmNA#26 (10 nM) was added to either 10 μM L687, 20 μM GSK1702934A, or 30 μM CBD with or without 20 μM SKF96365 in the medium. After 24 h, the intracellular fluorescence intensities were analysed by flow cytometry. Data are shown as the relative MFI of ASO in DMSO. All data are presented as mean ± standard error of the mean (SEM) of three independent experiments ( n = 3). Statistical significance was determined by comparing with values of DMSO using Tukey's test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ( B ) siRNA-mediated knockdown of the TRPC3/C6 channels. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed and compared with that in the untreated cells. ( C ) siRNA-mediated knockdown of TRPC3/C6 channel. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed by western blot and compared with that in untreated cells. ( D ) The effects of siRNA-mediated TRPC3/C6 channel knockdown on ASO uptake. TRPC3 siRNA (30 nM), TRPC6 (10 nM), or a combination of both were transfected into A549 cells for 48 h. The medium was replaced with Alexa647-AmNA#26 containing L687, and intracellular fluorescence intensities were analysed after 24 h. Data are shown as the relative MFI of ASO in DMSO. ( E ) Analysis of ASO uptake after incubating cells with a Ca 2+ chelator (BAPTA-AM). ASO and L687 were added to the medium, with or without 10 μM BAPTA-AM. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( F ) Analysis of dextran uptake mediated by L687. Alexa647-labelled dextran (1 and 3 μM) with 10 and 30 μM L687 was added to the medium, and A549 cells were cultured for 24 h. Intracellular fluorescence was analysed by flow cytometry, and the relative MFI was compared with 1 μM of Alexa647-dextran with DMSO. ( G ) Analysis of ASO uptake by incubating the cells with a macropinocytosis inhibitor (Cytochalasin D). L687 (30 μM) was then added to the medium. The following day, cells were then washed twice with PBS and incubated with cytochalasin D in the medium for 1 h. Then, cells were washed twice with PBS and incubated with Alexa647-AmNA#26 (10 nM) and L687 (30 μM). After 4 h, intracellular fluorescence intensities were analysed by flow cytometry. ( H ) Analysis of ASO uptake by incubating cells with a macropinocytosis inhibitor (EIPA). ASO and L687 were added to the medium with or without 100 μM EIPA. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( I ) Fluorescence imaging analysis of ASO incorporated into cells. Alexa647-AmNA#26 (100 nM) and L687 (30 μM) were added to the medium, and staining with Lysotracker-green and Hoechst, fluorescence microscopy imaging, and image analysis were performed after 48 h. ASO, antisense oligonucleotide; CBD, cannabidiol; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; TRPC, transient receptor potential canonical.

    Journal: Nucleic Acids Research

    Article Title: A novel transient receptor potential C3/C6 selective activator induces the cellular uptake of antisense oligonucleotides

    doi: 10.1093/nar/gkae245

    Figure Lengend Snippet: Effects of the TRPC3/C6/C7 inhibitor, knockdown of TRPC3/C6, and Ca 2+ chelator on ASO uptake, and examination of L687-mediated uptake pathways. ( A ) ASO uptake was analysed by incubating cells with or without a TRPC inhibitor (SKF96365). Alexa647-AmNA#26 (10 nM) was added to either 10 μM L687, 20 μM GSK1702934A, or 30 μM CBD with or without 20 μM SKF96365 in the medium. After 24 h, the intracellular fluorescence intensities were analysed by flow cytometry. Data are shown as the relative MFI of ASO in DMSO. All data are presented as mean ± standard error of the mean (SEM) of three independent experiments ( n = 3). Statistical significance was determined by comparing with values of DMSO using Tukey's test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ( B ) siRNA-mediated knockdown of the TRPC3/C6 channels. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed and compared with that in the untreated cells. ( C ) siRNA-mediated knockdown of TRPC3/C6 channel. TRPC3 siRNA (30 nM) or TRPC6 siRNA (10 nM) was transfected into A549 cells using Lipofectamine3000, and cells were collected after 48 h. The relative expression of TRPC3/C6 was analysed by western blot and compared with that in untreated cells. ( D ) The effects of siRNA-mediated TRPC3/C6 channel knockdown on ASO uptake. TRPC3 siRNA (30 nM), TRPC6 (10 nM), or a combination of both were transfected into A549 cells for 48 h. The medium was replaced with Alexa647-AmNA#26 containing L687, and intracellular fluorescence intensities were analysed after 24 h. Data are shown as the relative MFI of ASO in DMSO. ( E ) Analysis of ASO uptake after incubating cells with a Ca 2+ chelator (BAPTA-AM). ASO and L687 were added to the medium, with or without 10 μM BAPTA-AM. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( F ) Analysis of dextran uptake mediated by L687. Alexa647-labelled dextran (1 and 3 μM) with 10 and 30 μM L687 was added to the medium, and A549 cells were cultured for 24 h. Intracellular fluorescence was analysed by flow cytometry, and the relative MFI was compared with 1 μM of Alexa647-dextran with DMSO. ( G ) Analysis of ASO uptake by incubating the cells with a macropinocytosis inhibitor (Cytochalasin D). L687 (30 μM) was then added to the medium. The following day, cells were then washed twice with PBS and incubated with cytochalasin D in the medium for 1 h. Then, cells were washed twice with PBS and incubated with Alexa647-AmNA#26 (10 nM) and L687 (30 μM). After 4 h, intracellular fluorescence intensities were analysed by flow cytometry. ( H ) Analysis of ASO uptake by incubating cells with a macropinocytosis inhibitor (EIPA). ASO and L687 were added to the medium with or without 100 μM EIPA. After 24 h, intracellular fluorescence intensities were analysed by flow cytometry. ( I ) Fluorescence imaging analysis of ASO incorporated into cells. Alexa647-AmNA#26 (100 nM) and L687 (30 μM) were added to the medium, and staining with Lysotracker-green and Hoechst, fluorescence microscopy imaging, and image analysis were performed after 48 h. ASO, antisense oligonucleotide; CBD, cannabidiol; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; TRPC, transient receptor potential canonical.

    Article Snippet: Incubation with primary polyclonal rabbit anti-TRPC3 antibody (#ACC-016; Alomone Labs, Jerusalem, Israel), primary polyclonal rabbit anti-TRPC6 antibody (#ACC-120; Alomone Labs, Jerusalem, Israel) at 1:2000 dilution and mouse monoclonal anti-GAPDH (#AM4300; Thermo Fisher Scientific, MA, USA) at 1:2000 dilution was performed at 4°C overnight.

    Techniques: Knockdown, Fluorescence, Flow Cytometry, Transfection, Expressing, Western Blot, Cell Culture, Incubation, Imaging, Staining, Microscopy